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Article Dans Une Revue Journal of Cell Science Année : 2004

Inhibition of PrPSc formation by lentiviral gene transfer of PrP containing dominant negative mutations.

Résumé

Currently, there is no treatment to cure transmissible spongiform encephalopathies. By taking advantage of the 'prion-resistant' polymorphisms Q171R and E219K that naturally exist in sheep and humans, respectively, we have evaluated a therapeutic approach of lentiviral gene transfer. Here, we show that VSV-G (vesicular stomatitis virus G glycoprotein) pseudotyped FIV-(feline immunodeficiency virus) derived vectors carrying the mouse Prnp gene in which these mutations have been inserted, are able to inhibit prion replication in chronically prion-infected cells. Because lentiviral tools are able to transduce post-mitotic cells such as neurons or cells of the lymphoreticular system, this result might help the development of gene- or cell-therapy approaches to prion disease.
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Dates et versions

inserm-00143203 , version 1 (26-04-2007)

Identifiants

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Carole Crozet, Yea-Lih Lin, Clément Mettling, Chantal Mourton-Gilles, Pierre Corbeau, et al.. Inhibition of PrPSc formation by lentiviral gene transfer of PrP containing dominant negative mutations.. Journal of Cell Science, 2004, 117 (Pt 23), pp.5591-7. ⟨10.1242/jcs.01484⟩. ⟨inserm-00143203⟩
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