Analysis of Native Biological Surfaces Using a 100 kV Massive Gold Cluster Source

F.A. Fernandez-Lima , J. Post , J.D. Debord , M.J. Eller , S. V. Verkhoturov , S. Della-Negra (1) , A. S. Woods , E. A. Schweikert

1 IPNO - Institut de Physique Nucléaire d'Orsay

In the present work, the advantages of a new, 100 kV platform equipped with a massive gold cluster source for the analysis of native biological surfaces are shown. Inspection of the molecular ion emission as a function of projectile size demonstrates a secondary ion yield increase of 100× for 520 keV Au4004+ as compared to 130 keV Au31+ and 43 keV C60. In particular, yields of tens of percent of molecular ions per projectile impact for the most abundant components can be observed with the 520 keV Au4004+ probe. A comparison between 520 keV Au4004+ time-of-flight-secondary ion mass spectrometry (TOF-SIMS) and matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS) data showed a similar pattern and similar relative intensities of lipid components across a rat brain sagittal section. The abundant secondary ion yield of analyte-specific ions makes 520 keV Au4004+ projectiles an attractive probe for submicrometer molecular mapping of native surfaces.

Domaines: Chimie-Physique [physics.chem-ph]

F.A. Fernandez-Lima, J. Post, J.D. Debord, M.J. Eller, S. V. Verkhoturov, et al.. Analysis of Native Biological Surfaces Using a 100 kV Massive Gold Cluster Source. Analytical Chemistry, 2011, 82, pp.8448-8453. ⟨10.1021/ac201481r⟩. ⟨in2p3-00647222⟩