Impact of inoculation with the phytostimulatory PGPR Azospirillum lipoferum CRT1 on the genetic structure of the rhizobacterial community of field-grown maize

Ezekiel Baudoin (1, 2) , Sylvie Nazaret (1) , Christophe Mougel (3) , Lionel Ranjard (3) , Yvan Moenne-Loccoz (1)

1 LEM - Laboratoire d'Ecologie Microbienne - UMR 5557
2 SEQBIO - Séquestration du carbone et bio-fonctionnement des sols : effets des modes de gestion des agro-écosystèmes tropicaux
3 MSE - Microbiologie du Sol et de l'Environnement

The phytostimulatory PGPR Azospirillum lipoferum CRT1 was inoculated to maize seeds and the impact on the genetic structure of the rhizobacterial community in the field was determined during maize growth by Automated Ribosomal Intergenic Spacer Analysis (ARISA) of rhizosphere DNA extracts. ARISA fingerprints could differ from one plant to the next as well as from one sampling to the next. Inoculation with strain CRT1 enhanced plant-to-plant variability of the ARISA fingerprints and caused a statistically significant shift in the composition of the indigenous rhizobacterial community at the first two samplings. This is the first study on the ecological impact of Azospirillum inoculation on resident bacteria done in the field and showing that this impact can last at least one month

Mots clés: Azospirillum Rhizosphere ARISA Fingerprint Bacterial community Impact

Domaines: Biodiversité et Ecologie; Biochimie, Biologie Moléculaire; Science des sols

Ezekiel Baudoin, Sylvie Nazaret, Christophe Mougel, Lionel Ranjard, Yvan Moenne-Loccoz. Impact of inoculation with the phytostimulatory PGPR Azospirillum lipoferum CRT1 on the genetic structure of the rhizobacterial community of field-grown maize. Soil Biology and Biochemistry, 2009, 41 (2), pp.409-413. ⟨10.1016/j.soilbio.2008.10.015⟩. ⟨halsde-00525716⟩