Skip to Main content Skip to Navigation
Journal articles

Bacterial taxa associated with the hematophagous mite Dermanyssus gallinae detected by 16S rRNA PCR amplification and TTGE fingerprinting

Abstract : Dermanyssus gallinae (Arthropoda, Mesostigmata) is suspected to be involved in the transmission of a wide variety of pathogens, but nothing is known about its associated non-pathogenic bacterial community. To address this question, we examined the composition of bacterial communities in D. gallinae collected from standard poultry farms in Brittany, France. Genetic fingerprints of bacterial communities were generated by temporal temperature gradient gel electrophoresis (TTGE) separation of individual polymerase chain reaction (PCR)-amplified 16S rRNA gene fragments, followed by DNA sequence analysis. Most of the sequences belonged to the Proteobacteria and Firmicute phyla, with a majority of sequences corresponding to the Enterobacteriales order and the Staphylococcus genus. By using statistical analysis, we showed differences in biodiversity between poultry farms. We also determined the major phylotypes that compose the characteristic microbiota associated with D. gallinae. Saprophytes, opportunistic pathogens and pathogenic agents such as Pasteurella multocida, Erysipelothrix rhusiopathiae and sequences close to the genus Aerococcus were identified. Endosymbionts such as Schineria sp., Spiroplasma sp. Anistosticta, ''Candidatus Cardinium hertigii'' and Rickettsiella sp. were also present in the subdominant bacterial community. Identification of potential targets within the symbiont community may be considered in the future as a means of ectoparasite control.
Complete list of metadata

https://hal.archives-ouvertes.fr/halsde-00354551
Contributor : Christine Delorme <>
Submitted on : Tuesday, January 20, 2009 - 11:23:57 AM
Last modification on : Tuesday, July 20, 2021 - 5:20:06 PM

Identifiers

Citation

Claire Valiente Moro, Jean Thioulouse, Claude Chauve, Philippe Normand, Lionel Zenner. Bacterial taxa associated with the hematophagous mite Dermanyssus gallinae detected by 16S rRNA PCR amplification and TTGE fingerprinting. Research in Microbiology, Elsevier, 2008, 160 (1), pp.63-70. ⟨10.1016/j.resmic.2008.10.006⟩. ⟨halsde-00354551⟩

Share

Metrics

Record views

380