Response of Aedes aegypti (Diptera : Culicidae) larvae to three xenobiotic exposures: Larval tolerance and detoxifying enzyme activities

S. Boyer (1) , J. P. David (2) , D. Rey (3) , G. Lemperiere (1) , P. Ravanel (1)

1 LECA - Laboratoire d'Ecologie Alpine
2 Vector Research Group
3 Entente Interdépartementale pour la Démoustication

The ability of mosquito larvae to tolerate toxic compounds (temephos, Bacillus thuringiensis var. israelensis, toxic vegetable leaf litter) was examined on a laboratory larval strain of Aedes aegypti L. Bioassays and detoxifying enzyme activity measurements were performed to compare tolerance/resistance capacities. The possibility of a functional plasticity of detoxifying equipment was investigated through experimental determination of the inductive effect of each xenobiotic within a given generation. In the same way, the selective effect of a toxic leaf litter was also investigated along successive generations. Results revealed that differential cytochrome P450 monooxygenase, esterase, and glutathione S-transferase activity levels correlated with the bioassay results. Both induction and selection increased larval tolerance to the xenobiotics used and increased the levels of larval detoxifying enzyme activities.

Mots clés: mosquito larval tolerance; functional plasticity; Bacillus; thuringiensis var. israelensis; temephos; toxic leaf litter; Glutathione s-transferases; insecticide resistance; leaf-litter; drosophila-melanogaster; cytochromes p450; mosquito; toxicity; identification; induction; allelochemicals

Domaines: Biodiversité et Ecologie; Biodiversité; Ecologie, Environnement

S. Boyer, J. P. David, D. Rey, G. Lemperiere, P. Ravanel. Response of Aedes aegypti (Diptera : Culicidae) larvae to three xenobiotic exposures: Larval tolerance and detoxifying enzyme activities. Environmental Toxicology and Chemistry, 2006, 25 (2), pp.470-476. ⟨10.1897/05-267R2.1⟩. ⟨halsde-00293860⟩