Skip to Main content Skip to Navigation
Journal articles

Bacteriophage Prevalence in the Genus Azospirillum and Analysis of the First Genome Sequence of an Azospirillum brasilense Integrative Phage

Abstract : The prevalence of bacteriophages was investigated in 24 strains of four species of plant growth-promoting rhizobacteria belonging to the genus Azospirillum. Upon induction by mitomycin C, the release of phage particles was observed in 11 strains from three species. Transmission electron microscopy revealed two distinct sizes of particles, depending on the identity of the Azospirillum species, typical of the Siphoviridae family. Pulsed-field gel electrophoresis and hybridization experiments carried out on phage-encapsidated DNAs revealed that all phages isolated from A. lipoferum and A. doebereinerae strains had a size of about 10 kb whereas all phages isolated from A. brasilense strains displayed genome sizes ranging from 62 to 65 kb. Strong DNA hybridizing signals were shown for most phages hosted by the same species whereas no homology was found between phages harbored by different species. Moreover, the complete sequence of the A. brasilense Cd bacteriophage (Ab-Cd) genome was determined as a double-stranded DNA circular molecule of 62,337 pb that encodes 95 predicted proteins. Only 14 of the predicted proteins could be assigned functions, some of which were involved in DNA processing, phage morphogenesis, and bacterial lysis. In addition, the Ab-Cd complete genome was mapped as a prophage on a 570-kb replicon of strain A. brasilense Cd, and a region of 27.3 kb of Ab-Cd was found to be duplicated on the 130-kb pRhico plasmid previously sequenced from A. brasilense Sp7, the parental strain of A. brasilense Cd.
Complete list of metadata

https://hal.archives-ouvertes.fr/halsde-00259504
Contributor : Christine Delorme Connect in order to contact the contributor
Submitted on : Thursday, February 28, 2008 - 1:06:06 PM
Last modification on : Monday, December 13, 2021 - 9:14:33 AM

Identifiers

Citation

Mickaël Boyer, Jacqueline Haurat, Sylvie Samain, Béatrice Segurens, Frédérick Gavory, et al.. Bacteriophage Prevalence in the Genus Azospirillum and Analysis of the First Genome Sequence of an Azospirillum brasilense Integrative Phage. Applied and Environmental Microbiology, American Society for Microbiology, 2008, 74 (3), pp.861-874. ⟨10.1128/AEM.02099-07⟩. ⟨halsde-00259504⟩

Share

Metrics

Les métriques sont temporairement indisponibles