Localization of a human nucleoporin 155 gene (NUP155) to the 5p13 region and cloning of its cDNA

Xiuqing Zhang , Huanming Yang , Morten J. Corydon , Xiaoxiao Zhang , Soren Pedersen , Julie R. Korenberg , Xiao-Ning Chen , Jocelyn Laporte (1, 2) , Niels Gregersen , Erik Niebuhr , Guoyang Liu , Lars Bolund

1 IGBMC - Institut de génétique et biologie moléculaire et cellulaire
2 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire

Nucleoporins are the main components of nuclear pore complexes (NPCs) involved in nucleo-cytoplasmic transport. Starting with an expressed DNA fragment retrieved by exon trapping from pooled human BAC clones mapped to the short arm of chromosome 5, we identified a human nucleoporin cDNA sequence by PCR from a human testis cDNA library. The coding sequence showed high homology to that of the rat nucleoporin 155 (Nup155) cDNA. FISH analysis with the human BAC clone as probe localized the human NUP155 gene to chromosome band 5p13. Northern analysis showed that the human NUP155 gene was expressed at different levels in all tissues tested. Two species of transcripts were observed with estimated lengths of 5.4 and 4.7 kb, respectively, in concordance with the finding of two alternative polyadenylation sites in the cDNA. The genomic location of the human NUP155 gene suggests a possible role in the mental and developmental retardation associated with hemizygous deletions of the 5p13 region.

Domaines: Biochimie, Biologie Moléculaire

Xiuqing Zhang, Huanming Yang, Morten J. Corydon, Xiaoxiao Zhang, Soren Pedersen, et al.. Localization of a human nucleoporin 155 gene (NUP155) to the 5p13 region and cloning of its cDNA. Genomics, 1999, 57 (1), pp.144-151. ⟨10.1006/geno.1999.5741⟩. ⟨hal-04033187⟩