Gene targeting restricted to mouse striated muscle lineage

Pierre Miniou (1) , Danilo Tiziano (1) , Tony Frugier (1) , Natacha Roblot (1) , Marianne Le Meur (1) , Judith Melki (1)

1 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire

Spatially and temporally regulated somatic mutations can be achieved by using the Cre/LoxP recombination system of bacteriophage P1. In order to develop gene knockouts restricted to striated muscle, we generated a transgenic mouse line expressing Cre recombinase under the control of the human α-skeletal actin promoter. Specific excision of a loxP-flanked gene was demonstrated in striated muscle, heart and skeletal muscle, in a pattern very similar to the expression of the endogenous α-skeletal actin gene. Therefore, the reported transgenic line can be used to target inactivation or activation of a given gene to the skeletal muscle lineage.

Domaines: Biochimie, Biologie Moléculaire

Pierre Miniou, Danilo Tiziano, Tony Frugier, Natacha Roblot, Marianne Le Meur, et al.. Gene targeting restricted to mouse striated muscle lineage. Nucleic Acids Research, 1999, 27 (19), pp.27-30. ⟨10.1093/nar/27.19.e27⟩. ⟨hal-04026890⟩