Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors

Adam M Thomas (1) , Sanja Brolih (1) , Joanna F Mcgouran (1) , Afaf H El-Sagheer (1) , Denis Ptchelkine (1) , Morgan Jones (2) , Neil Q Mcdonald (2) , Peter J Mchugh (1) , Tom Brown (1)

1 University of Oxford
2 The Francis Crick Institute [London]

We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery.

Domaines: Sciences du Vivant [q-bio]

Adam M Thomas, Sanja Brolih, Joanna F Mcgouran, Afaf H El-Sagheer, Denis Ptchelkine, et al.. Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors. Chemical Communications, 2019, 55, pp.11671 - 11674. ⟨10.1039/c9cc05476f⟩. ⟨hal-04019891⟩