Cloning and characterization of GETS-1, a goldfish Ets family member that functions as a transcriptional repressor in muscle - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochemical Journal Année : 1998

Cloning and characterization of GETS-1, a goldfish Ets family member that functions as a transcriptional repressor in muscle

Daniel Goldman
  • Fonction : Auteur
Mohan K. Sapru
  • Fonction : Auteur
Scott Stewart
  • Fonction : Auteur
Joshua Plotkin
  • Fonction : Auteur
Towia A. Libermann
  • Fonction : Auteur
Kunliang Guan
  • Fonction : Auteur

Résumé

An Ets transcription factor family member, GETS-1, was cloned from a goldfish retina cDNA library. GETS-1 contains a conserved Ets DNA-binding domain at its N-terminus and is most similar to ternary complex factor (TCF) serum-response-factor protein-1a (SAP-1a). GETS-1 is expressed in many tissues, but is enriched in retina and brain. As with the TCFs SAP-1a and ets-related protein (ERP), overexpression of the GETS-1 promoter suppresses nicotinic acetylcholine receptor epsilon-subunit gene expression in cultured muscle cells. A consensus Ets binding site sequence in the promoter of the epsilon-subunit gene is required for GETS-1-mediated repression. GETS-1 repressor activity is abrogated by overexpression of an activated Ras/mitogen-activated protein kinase (MAP kinase) or by mutation of Ser-405, a MAP kinase phosphorylation site in GETS-1. Fusion proteins created between GETS-1 and the Gal4 DNA-binding domain show that, like other TCFs, GETS-1 contains a C-terminal activation domain that is activated by a Ras/MAP kinase signalling cascade. Interestingly, mutation of Ser-405 located within this activation domain abrogated transcriptional activation of the fusion protein.

Dates et versions

hal-03991585 , version 1 (15-02-2023)

Identifiants

Citer

Daniel Goldman, Mohan K. Sapru, Scott Stewart, Joshua Plotkin, Towia A. Libermann, et al.. Cloning and characterization of GETS-1, a goldfish Ets family member that functions as a transcriptional repressor in muscle. Biochemical Journal, 1998, 335 (2), pp.267-275. ⟨10.1042/bj3350267⟩. ⟨hal-03991585⟩
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