Actin Architecture Steers Microtubules in Active Cytoskeletal Composite

Ondřej Kučera (1) , Jérémie Gaillard (1) , Christophe Guérin (1) , Clothilde Utzschneider (1) , Manuel Théry (1) , Laurent Blanchoin (1)

1 CytoMorphoLab

Motility assays use surface-immobilized molecular motors to propel cytoskeletal filaments. They have been widely used to characterize motor properties and their impact on cytoskeletal self-organization. Moreover, the motility assays are a promising class of bioinspired active tools for nanotechnological applications. While these assays involve controlling the filament direction and speed, either as a sensory readout or a functional feature, designing a subtle control embedded in the assay is an ongoing challenge. Here, we investigate the interaction between gliding microtubules and networks of actin filaments. We demonstrate that the microtubule's behavior depends on the actin architecture. Both unbranched and branched actin decelerate microtubule gliding; however, an unbranched actin network provides additional guidance and effectively steers the microtubules. This effect, which resembles the recognition of cortical actin by microtubules, is a conceptually new means of controlling the filament gliding with potential application in the design of active materials and cytoskeletal nanodevices.

Mots clés: cytoskeletal composite; gliding assay; microtubules; network architecture; actin filaments

Domaines: Biologie cellulaire

Ondřej Kučera, Jérémie Gaillard, Christophe Guérin, Clothilde Utzschneider, Manuel Théry, et al.. Actin Architecture Steers Microtubules in Active Cytoskeletal Composite. Nano Letters, 2022, 22 (21), pp.8584-8591. ⟨10.1021/acs.nanolett.2c03117⟩. ⟨hal-03857728⟩