Functional genomics uncovers the transcription factor BNC2 as required for myofibroblastic activation in fibrosis

Marie Bobowski-Gerard (1) , Clémence Boulet , Francesco Zummo , Julie Dubois-Chevalier , Céline Gheeraert , Mohamed Bou Saleh , Jean-Marc Strub , Amaury Farce , Maheul Ploton , Loïc Guille , Jimmy Vandel , Antonino Bongiovanni , Ninon Very , Eloïse Woitrain , Audrey Deprince , Fanny Lalloyer , Eric Bauge , Lise Ferri , Line-Carolle Ntandja-Wandji , Alexia Cotte , Corinne Grangette , Emmanuelle Vallez , Sarah Cianférani , Violeta Raverdy , Robert Caiazzo , Viviane Gnemmi , Emmanuelle Leteurtre (2, 3) , Benoit Pourcet , Réjane Paumelle , Kim Ravnskjaer , Guillaume Lassailly , Joel Haas , Philippe Mathurin , François Pattou , Laurent Dubuquoy , Bart Staels , Philippe Lefebvre , Jérôme Eeckhoute (4, 5, 6, 7, 1)

1 RNMCD - Récepteurs Nucléaires, Maladies Métaboliques et Cardiovasculaires - U1011
2 Institut de Pathologie [CHU Lille]
3 CANTHER - Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277
4 Université de Lille
5 Institut Pasteur de Lille
6 INSERM - Institut National de la Santé et de la Recherche Médicale
7 CHU de Lille - Centre Hospitalier Universitaire de Lille

Abstract Tissue injury triggers activation of mesenchymal lineage cells into wound-repairing myofibroblasts, whose unrestrained activity leads to fibrosis. Although this process is largely controlled at the transcriptional level, whether the main transcription factors involved have all been identified has remained elusive. Here, we report multi-omics analyses unraveling Basonuclin 2 (BNC2) as a myofibroblast identity transcription factor. Using liver fibrosis as a model for in-depth investigations, we first show that BNC2 expression is induced in both mouse and human fibrotic livers from different etiologies and decreases upon human liver fibrosis regression. Importantly, we found that BNC2 transcriptional induction is a specific feature of myofibroblastic activation in fibrotic tissues. Mechanistically, BNC2 expression and activities allow to integrate pro-fibrotic stimuli, including TGFβ and Hippo/YAP1 signaling, towards induction of matrisome genes such as those encoding type I collagen. As a consequence, Bnc2 deficiency blunts collagen deposition in livers of mice fed a fibrogenic diet. Additionally, our work establishes BNC2 as potentially druggable since we identified the thalidomide derivative CC-885 as a BNC2 inhibitor. Altogether, we propose that BNC2 is a transcription factor involved in canonical pathways driving myofibroblastic activation in fibrosis.

Domaines: Sciences du Vivant [q-bio]

Marie Bobowski-Gerard, Clémence Boulet, Francesco Zummo, Julie Dubois-Chevalier, Céline Gheeraert, et al.. Functional genomics uncovers the transcription factor BNC2 as required for myofibroblastic activation in fibrosis. Nature Communications, 2022, 13 (1), pp.5324. ⟨10.1038/s41467-022-33063-9⟩. ⟨hal-03774786⟩