Autofluorescence Loss in Photobleaching for Human Dentin ex vivo

Seunghwan Goldmund Lee (1, 2) , Minwoo Kim (2) , Sunghee Jeong (2) , Jaejoon Hwang (2) , Jisu Kim (2) , Aurélien Gourrier (1) , Jean-Claude Vial (1) , Kwangseuk Kyhm (2)

1 LIPhy - Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères]
2 PNU - Pusan National University

Two-photon fluorescence microscopy was performed on the enamel-dentin junction area of a human tooth using a femtosecond pulsed laser. We obtained a clear image contrast between the bright dentin and dark tubules with the autofluorescence generated from the endogenous fluorophores in dentin. The autofluorescence shows a broad spectrum due to complex cross links between dentinal collagens, which extend from blue to orange wavelengths (470–590 nm), but a gradual autofluorescence loss in pho- tobleaching was observed for a long-term exposure under strong excitation. For increasing excitation power, we found that two-step decay becomes significant in the spectrally integrated autofluorescence.

Mots clés: Autofluorescence; Dentin; Fluorescence loss in photobleaching; Two-photon fluorescence microscopy

Domaines: Anatomie, Histologie, Anatomopathologie [q-bio.TO]; Biophysique [physics.bio-ph]; Optique [physics.optics]

Seunghwan Goldmund Lee, Minwoo Kim, Sunghee Jeong, Jaejoon Hwang, Jisu Kim, et al.. Autofluorescence Loss in Photobleaching for Human Dentin ex vivo. Current Optics and Photonics, 2022, 6 (1), pp.86-91. ⟨hal-03704166⟩