Non-monotonous enzyme-assisted self-assembly profiles resulting from reaction-diffusion processes in host gels - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Colloid and Interface Science Année : 2022

Non-monotonous enzyme-assisted self-assembly profiles resulting from reaction-diffusion processes in host gels

Jean-Yves Runser
Miryam Criado Gonzalez
Fatima Fneich
  • Fonction : Auteur
Morgane Rabineau
  • Fonction : Auteur
  • PersonId : 1116028
Bernard Senger
  • Fonction : Auteur
  • PersonId : 1116018
Pierre Weiss
  • Fonction : Auteur
Loïc Jierry
Pierre Schaaf

Résumé

Reaction–diffusion (RD) processes are responsible for surface and in-depth micropatterning in inanimate and living matter. Here we show that enzyme-assisted self-assembly (EASA) of peptides is a valuable tool to functionnalize host gels. By using a phosphatase distributed in a host hydrogel, the diffusion of phosphorylated peptides from a liquid/host gel interface leads to the spontaneous formation of a pattern of dephosphorylated peptide self-assembly presenting at least two self-assembly maxima. Variation of enzyme and peptide concentrations change the pattern characteristics. When a peptide drop is deposited on a phosphatase functionalized gel, a self-assembly pattern is also formed both along the gel-solution interface and perpendicular to the interface. This self-assembly pattern induces a local change of the gel mechanical properties measured by nanoindentation. Its appearance relies on the formation of self-assembled structures by nucleation and growth processes which are static in the hydrogel. This process presents great similarities with the Liesegang pattern formation and must be taken into account for the functionalization of hydrogels by EASA. A mechanism based on RD is proposed leading to an effective mathematical model accounting for the pattern formation. This work highlights EASA as a tool to design organic Liesegang-like microstructured materials with potential applications in biomaterials and artificial living systems design.

Domaines

Autre [q-bio.OT]

Dates et versions

hal-03670935 , version 1 (17-05-2022)

Identifiants

Citer

Jean-Yves Runser, Miryam Criado Gonzalez, Fatima Fneich, Morgane Rabineau, Bernard Senger, et al.. Non-monotonous enzyme-assisted self-assembly profiles resulting from reaction-diffusion processes in host gels. Journal of Colloid and Interface Science, 2022, 620, pp.234-241. ⟨10.1016/j.jcis.2022.03.150⟩. ⟨hal-03670935⟩
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