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Study of electrochemotherapy effect in cell spheroids using a microfluidic device based on glass technology

Abstract : Electroporation (EPN) is a technique using pulsed electric fields to permeabilize and introduce molecules into cells without permanent damage. To assess the potential of cancer treatments based on EPN, like electrochemo- therapy (ECT), innovative in vitro assays exploiting 3D cell models such as spheroids are needed. The parallel treatment of tens of spheroids is required to produce statistical data. We designed a microfluidic device including a structured hydrogel placed between two conductive glass slides, enabling culture and electroporation of a large number of spheroids sharing similar characteristics. Here, we demonstrate its use to assess the effect of ECT on spheroids.
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https://hal.archives-ouvertes.fr/hal-03661913
Contributor : Marie FRENEA-ROBIN Connect in order to contact the contributor
Submitted on : Sunday, May 8, 2022 - 5:39:06 PM
Last modification on : Saturday, September 24, 2022 - 2:30:05 PM

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  • HAL Id : hal-03661913, version 1

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Pauline Bregigeon, Julien Marchalot, Laure Franqueville, Christian Vollaire, Marie Frénéa-Robin, et al.. Study of electrochemotherapy effect in cell spheroids using a microfluidic device based on glass technology. 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021), Oct 2021, Palm Springs, California, United States. ⟨hal-03661913⟩

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