Listeriolysin S: A bacteriocin from Listeria monocytogenes that induces membrane permeabilization in a contact-dependent manner

Jazmín Meza-Torres (1, 2, 3, 4) , Mickaël Lelek (5) , Juan Quereda (6) , Martin Sachse (7) , Giulia Manina (8) , Dmitry Ershov (9, 10) , Jean-Yves Tinevez (9) , Lilliana Radoshevich (11) , Claire Maudet (12) , Thibault Chaze (13) , Quentin Giai Gianetto (13, 10) , Mariette I Matondo (13) , Marc Lecuit (12, 3, 4, 14) , Isabelle Martin-Verstraete (15) , Christophe Zimmer (5) , Hélène Bierne (16, 17) , Olivier Dussurget (1, 3, 4) , Pascale Cossart (2) , Javier Pizarro-Cerdá (18)

1 Yersinia
2 Interactions Bactéries-Cellules
3 UPCité - Université Paris Cité
4 USPC - Université Sorbonne Paris Cité
5 Imagerie et Modélisation - Imaging and Modeling
6 CEU-UCH - Universidad Cardenal Herrera-CEU
7 UTechS UBI - Plateforme BioImagerie Ultrastructurale – Ultrastructural BioImaging Platform
8 Individualité microbienne et infection - Microbial Individuality and Infection
9 IAH - Hub d'analyse d'images - Image Analysis Hub (Platform)
10 Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB
11 University of Iowa [Iowa City]
12 Biologie des Infections - Biology of Infection
13 UTechS MSBio - Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology
14 Service des Maladies infectieuses et tropicales [CHU Necker]
15 PBA (U-Pasteur_6) - Pathogénèse des Bactéries Anaérobies / Pathogenesis of Bacterial Anaerobes
16 INRAE - Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement
17 Université Paris-Saclay
18 Yersinia

Significance Listeria monocytogenes ( Lm ) is a bacterial pathogen that causes listeriosis, a foodborne disease characterized by gastroenteritis, meningitis, bacteremia, and abortions in pregnant women. The most severe human listeriosis outbreaks are associated with a subset of Lm hypervirulent clones that encode the bacteriocin Listeriolysin S (LLS), which modifies the gut microbiota and allows efficient Lm gut colonization and invasion of deeper organs. Our present work identifies the killing mechanism displayed by LLS to outcompete gut commensal bacteria, demonstrating that it induces membrane permeabilization and membrane depolarization of target bacteria. Moreover, we show that LLS is a thiazole/oxazole–modified microcin that displays a contact-dependent inhibition mechanism.

Mots clés: microfluidic microscopy; contact-dependent inhibition (CDI); bacteriocin; Listeriolysin S (LLS); Listeria monocytogenes (Lm)

Domaines: Sciences du Vivant [q-bio]; Bactériologie

Jazmín Meza-Torres, Mickaël Lelek, Juan Quereda, Martin Sachse, Giulia Manina, et al.. Listeriolysin S: A bacteriocin from Listeria monocytogenes that induces membrane permeabilization in a contact-dependent manner. Proceedings of the National Academy of Sciences of the United States of America, 2021, 118 (40), pp.e2108155118. ⟨10.1073/pnas.2108155118⟩. ⟨hal-03620714⟩