Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells

Renata Santos (1, 2) , Arianna Mei (1) , Maria Marchetto (1, 3)

1 The Salk Institute for Biological Studies
2 IPNP - U1266 Inserm - Institut de psychiatrie et neurosciences de Paris
3 UC San Diego - University of California [San Diego]

Astrocytes are an essential component of the central nervous system for neuronal support and response to injury and disease. Here, we present a protocol to generate glial progenitor cells (GPCs) from human-induced pluripotent stem cells (iPSCs) that can be further differentiated into inflammation-responsive astrocytes. This two-step protocol has the advantage of reducing the time of astrocyte differentiation since GPCs can be frozen and stored.

Domaines: Sciences du Vivant [q-bio]

Renata Santos, Arianna Mei, Maria Marchetto. Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells. STAR Protocols, 2022, 3 (2), pp.101261. ⟨10.1016/j.xpro.2022.101261⟩. ⟨hal-03616279⟩