Fast optical recording of neuronal activity by three-dimensional custom-access serial holography

Walther Akemann (1) , Sébastien Wolf (1) , Vincent Villette (1) , Benjamin Mathieu (1) , Astou Tangara (1) , Jozsua Fodor (1) , Cathie Ventalon (1) , Jean-François Léger (1) , Stéphane Dieudonné (1) , Laurent Bourdieu (1)

1 IBENS - Institut de biologie de l'ENS Paris

Optical recording of neuronal activity in 3D brain circuits at cellular and millisecond resolution in-vivo is essential for probing information flow in the brain. While random-access multiphoton microscopy permits fast optical access to neuronal targets in 3D, the method is challenged by motion artefacts when recording from behaving animals. Therefore, we developed 3D custom-access serial holography (3D-CASH). Built upon a fast acousto-optic light modulator, 3D-CASH performs serial sampling at 40 kHz from neurons at freely selectable 3D locations. Motion artefacts are eliminated by targeting each neuron with a size-optimized pattern of excitation light covering the cell body and its anticipated displacement field. Spike rates inferred from GCaMP6f recordings in visual cortex of awake mice tracked the phase of a moving bar stimulus with higher spike correlation between intra-compared to inter-laminar neuron pairs. 3D-CASH offers access to the millisecond correlation structure of in-vivo neuronal activity in 3D microcircuits.

Domaines: Neurobiologie; Optique [physics.optics]

Walther Akemann, Sébastien Wolf, Vincent Villette, Benjamin Mathieu, Astou Tangara, et al.. Fast optical recording of neuronal activity by three-dimensional custom-access serial holography. Nature Methods, 2022, 19 (1), pp.100-110. ⟨10.1038/s41592-021-01329-7⟩. ⟨hal-03606564⟩