Optimized nested PCR enhances biological diagnosis and phylogenetic analysis of human parvovirus B19 infections

Maxime Pichon (1, 2, 3, 4) , Clément Labois (2, 3, 4) , Véronique Tardy-Guidollet (2, 5) , Delphine Mallet (2, 5) , Jean-Sébastien Casalegno (2, 3, 4, 1) , Geneviève Billaud (2, 3, 4) , Bruno Lina (2, 3, 4, 1) , Pascal Gaucherand (2, 6) , Yahia Mekki (2, 3, 4)

1 CIRI - Centre International de Recherche en Infectiologie
2 HCL - Hospices Civils de Lyon
3 IAI - Institut des Agents Infectieux [Lyon]
4 Centre de Biologie et Pathologie Nord [Hôpital de la Croix-Rousse, CHU-HCL]
5 Centre de Biologie et Pathologie Est [Hospices Civils de Lyon]
6 HFME - Hôpital Femme Mère Enfant [CHU - HCL]

Diagnosis and epidemiological analysis of human parvovirus B19 (hB19V) infections are essential for disease management in severely ill patients. This study aimed to evaluate the performance of an optimized NS1-VP1u nested PCR for detection and sequencing of viruses in clinical samples using 224 clinical and five reference samples. PCR sensitivity, specificity, and positive and negative predictive values were perfect (100%). While phylogenetic analysis of a 615 bp-long fragment demonstrated that the viruses in all of the samples belonged to genotype 1, this study confirmed that this optimized PCR could detect all known hB19V with high performance.

Domaines: Maladies infectieuses; Virologie; Santé publique et épidémiologie

Maxime Pichon, Clément Labois, Véronique Tardy-Guidollet, Delphine Mallet, Jean-Sébastien Casalegno, et al.. Optimized nested PCR enhances biological diagnosis and phylogenetic analysis of human parvovirus B19 infections. Archives of Virology, 2019, 164, pp.2775 - 2781. ⟨10.1007/s00705-019-04368-w⟩. ⟨hal-03591503⟩