Influence of homocysteine on the physical structure and molecular mobility of elastin network in cultured arteries

Valérie Samouillan (1) , Edouard Lamy , Jany Dandurand (1) , Alexandrine Foucault-Bertaud , Corinne Chareyre , Colette Lacabanne (1) , Philippe Charpiot

1 CIRIMAT - Centre interuniversitaire de recherche et d'ingenierie des matériaux

The thermal and dielectric properties of the elastin network were investigated in arteries cultured with physiological and pathological concentrations of homocysteine, an aminoacid responsible of histological impairments in human arteries. The glass transition of this amorphous protein was investigated by Differential Scanning Calorimetry (DSC). To explore the molecular dynamics of the elastin network in the nanometer range, we used Thermally Stimulated Currents (TSC), a dielectric technique running at low frequency and measuring the dipolar reorientations in proteins subjected to a static electrical field. Combining TSC and DSC experiments with determination of the activation parameters of relaxation times reveals the molecular mobility of the proteins. The major differences in the relaxation behavior of elastin between arteries cultured with physiological and pathological concentrations of homocysteine are discussed.

Mots clés: Thermally stimulated and depolarization current; Glass transition

Domaines: Matériaux

Valérie Samouillan, Edouard Lamy, Jany Dandurand, Alexandrine Foucault-Bertaud, Corinne Chareyre, et al.. Influence of homocysteine on the physical structure and molecular mobility of elastin network in cultured arteries. Journal of Non-Crystalline Solids, 2009, 3 (14-), pp.830-835. ⟨10.1016/j.jnoncrysol.2009.04.027⟩. ⟨hal-03571982⟩