Improved Productivity of NAD+ Reduction under Forced Convection in Aerated Solutions

Thi Xuan Huong Le (1) , Julius Gajdar (1) , Neus Vilà (1) , Alain Celzard (1) , Vanessa Fierro (2) , Alain Walcarius (1) , François Lapicque (3) , Mathieu Etienne (1)

1 LCPME - Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement
2 IJL - Institut Jean Lamour
3 LRGP - Laboratoire Réactions et Génie des Procédés

The regeneration of the NADH cofactor is crucial for bioelectrocatalytic reactors. This can be achieved using an electrochemical mediator such as the rhodium complex [Cp*Rh(bpy)Cl] + , but the overall reduction process suffers from the interference of molecular oxygen. This interference can be avoided by using a second porous working electrode (acting as a real oxygen filter) positioned near the surface of the first working electrode. To fabricate the oxygen filter, platinum particles were deposited on the surface of a carbon paper. NADH could be produced in the presence of the oxygen filter with a faradaic yield at 63.4%, very close to the yields obtained without the filter at 61.7% in a fully N2-degased medium, and only at 10.6% in the presence of oxygen. Moreover, the productivity was also increased by nearly a factor 4. Therefore, the proposed concept of the oxygen filter offers a new avenue in the current strategies for NADH regeneration in biocatalytic reactors based on electrochemical methods.

Domaines: Chimie théorique et/ou physique

Thi Xuan Huong Le, Julius Gajdar, Neus Vilà, Alain Celzard, Vanessa Fierro, et al.. Improved Productivity of NAD+ Reduction under Forced Convection in Aerated Solutions. ChemElectroChem, 2022, 9 (5), ⟨10.1002/celc.202101225⟩. ⟨hal-03562062⟩