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Article Dans Une Revue STAR Protocols Année : 2022

Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples

Résumé

Multiplexed single-cell RNA-sequencing (scRNA-seq) enables investigating several biological samples in one scRNA-seq experiment. Here, we use antibodies tagged with a hashtag oligonucleotide (HTO) to label each sample, and 103 Genomics technology to analyze singlecell gene expression. Advantages of sample multiplexing are to reduce the cost of scRNA-seq assay and to avoid batch effect. It may also facilitate cell-doublet removal and the merging of several scRNA-seq assays. Herein, we apply multiplexed scRNA-seq to investigate mouse thymocytes and splenic T lymphocytes development.
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Dates et versions

hal-03555152 , version 1 (03-02-2022)

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Saran Pankaew, Clémence Grosjean, Julie Quessada, Marie Loosveld, Delphine Potier, et al.. Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples. STAR Protocols, 2022, 3 (1), pp.101041. ⟨10.1016/j.xpro.2021.101041⟩. ⟨hal-03555152⟩
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