Endoplasmic reticulum stress and mineralization inhibition mechanism by the resinous monomer HEMA

E. Diamanti , S. Mathieu , C. Jeanneau , E. Kitraki , P. Panopoulos , G. Spyrou , I. About (1)

1 ISM - Institut des Sciences du Mouvement Etienne Jules Marey

Endoplasmic reticulum stress and mineralization inhibition mechanism by the resinous monomer HEMA. Aim To investigate the expression of two endoplasmic reticulum (ER)-resident key chaperone proteins, ERdj5 and BiP, under the influence of resinous monomers and its relationship with the inhibition of mineralization caused by the monomer 2-hydroxyethyl methacrylate (HEMA). Methodology The ERdj5 and BiP expression was studied in vitro, in primary human pulp cell cultures after treatment with three different HEMA concentrations at different time periods. Subsequently, the expression of both the odontoblast markers dentine sialoprotein (DSP) and osteonectin (OSN) was studied in human pulp cells under the same conditions. Results The ERdj5 and BiP expression was upregulated in the pulp cells. DSP and OSN were largely dispersed in the cytoplasm in control cell cultures but accumulated in a perinuclear area after exposure to HEMA. Their expression levels were not affected. Conclusions The increased expression of ERdj5 and BiP may reflect activation of ER stress. DSP and OSN accumulation into the cells may lead to their secretion arrest and inhibition of dentine matrix formation. These events may elucidate the mechanism by which HEMA inhibits the mineralization process.

Mots clés: BiP; dentine sialoprotein; endoplasmic reticulum stress; ERdj5; HEMA; mineralization; osteonectin; resinousmonomers

Domaines: Sciences du Vivant [q-bio]

E. Diamanti, S. Mathieu, C. Jeanneau, E. Kitraki, P. Panopoulos, et al.. Endoplasmic reticulum stress and mineralization inhibition mechanism by the resinous monomer HEMA. International Endodontic Journal, 2013, 46 (2), pp.160-168. ⟨10.1111/j.1365-2591.2012.02103.x⟩. ⟨hal-03551981⟩