Transcriptional Activators Stimulate DNA Repair

Philippe Frit (1) , Kyungrim Kwon (1) , Frédéric Coin (1) , Jérôme Auriol (1) , Sandy Dubaele (1) , Bernard Salles (1) , Jean-Marc Egly (1)

1 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire

To counteract the deleterious effects of genotoxic injury, cells have set up a sophisticated network of DNA repair pathways. We show that Gal4-VP16 and RAR transcriptional activators stimulate nucleotide excision repair (NER). This DNA repair activation is not coupled to transcription since it occurs in Cockayne syndrome cells (which are transcription-coupled repair deficient) and is observed in vitro in the presence of alpha-amanitin and in the absence of the basal transcription factors. Using a reconstituted dual incision assay, we also show that binding of activators to their cognate sequences induces a local chromatin remodeling mediated by ATP-driven chromatin remodeling and acetyltransferase activities to facilitate DNA repair.

Domaines: Sciences du Vivant [q-bio]

Philippe Frit, Kyungrim Kwon, Frédéric Coin, Jérôme Auriol, Sandy Dubaele, et al.. Transcriptional Activators Stimulate DNA Repair. Molecular Cell, 2002, 10 (6), pp.1391-1401. ⟨10.1016/s1097-2765(02)00732-3⟩. ⟨hal-03542314⟩