Lead-induced DNA damage in Vicia faba root cells: Potential involvement of oxidative stress

Bertrand Pourrut (1, 2) , Séverine Jean (1) , Jérôme Silvestre (1) , Eric Pinelli (1)

1 LEFE - Laboratoire Ecologie Fonctionnelle et Environnement
2 LGCgE - Laboratoire Génie Civil et Géo-Environnement [Béthune]

Genotoxic effects of lead (0–20 µM) were investigated in whole-plant roots of Vicia faba L., grown hydroponically under controlled conditions. Lead-induced DNA damage in V. faba roots was evaluated by use of the comet assay, which allowed the detection of DNA strand-breakage and with the V. faba micronucleus test, which revealed chromosome aberrations. The results clearly indicate that lead induced DNA fragmentation in a dose-dependant manner with a maximum effect at 10 µM. In addition, at this concentration, DNA damage time-dependently increased until 12 h. Then, a decrease in DNA damages was recorded. The significant induction of micronucleus formation also reinforced the genotoxic character of this metal. Direct interaction of lead with DNA was also evaluated with the a-cellular comet assay. The data showed that DNA breakages were not associated with a direct effect of lead on DNA. In order to investigate the relationship between lead genotoxicity and oxidative stress, V. faba were exposed to lead in the presence or absence of the antioxidant Vitamin E, or the NADPH-oxidase inhibitor dephenylene iodonium (DPI). The total inhibition of the genotoxic effects of lead (DNA breakage and micronucleus formation) by these compounds reveals the major role of reactive oxygen species (ROS) in the genotoxicity of lead. These results highlight, for the first time in vivo and in whole-plant roots, the relationship between ROS, DNA strand-breaks and chromosome aberrations induced by lead.

Mots clés: Micronucleus test; Reactive oxygen species; Comet assay; DNA breakages; Genotoxicity; Heavy metal

Domaines: Génie chimique; Biodiversité et Ecologie

Bertrand Pourrut, Séverine Jean, Jérôme Silvestre, Eric Pinelli. Lead-induced DNA damage in Vicia faba root cells: Potential involvement of oxidative stress. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 2011, 726 (2), pp.123-128. ⟨10.1016/j.mrgentox.2011.09.001⟩. ⟨hal-03541114⟩