Toward Whole Brain Label-free Molecular Imaging with Single-cell Resolution sing Ultra-broadband Multiplex CARS Microspectroscopy

Yusuke Murakami (1) , Shinichi Miyazaki (1) , Damien Boildieu (2, 3) , Zakaniaina Rajaofara (2, 3) , David Helbert (4, 5, 6) , Amandine Magnaudeix (7, 2, 8) , Philippe Carré (4, 5) , Philippe Leproux (3, 2) , Sakiko Honjoh (1) , Hideaki Kano (9)

1 Université de Tsukuba = University of Tsukuba
2 UNILIM - Université de Limoges
3 XLIM-PHOT - Photonique Fibre et Sources Cohérentes
4 UP - Université de Poitiers = University of Poitiers
5 XLIM-ASALI - Synthèse et analyse d'images
6 Université de Poitiers - Faculté de Sciences fondamentales et appliquées
7 IRCER-AXE4 - IRCER - Axe 4 : céramiques sous contraintes environnementales
8 IRCER - Institut de Recherche sur les CERamiques
9 Kyushu University

Mapping the distribution of chemical molecules throughout a brain is helpful for neuroscience research. We have applied an ultra-broadband multiplex CARS spectroscopic imaging system to construct a whole-brain label-free molecular map in macro and micro scales. We could precisely visualize lipids distributed to white matter, rich in neuronal fibers. Our microscale measurements figured out that cells within the hippocampus and cerebral cortex could be divided into lipid-rich and water-rich cells. Moreover, we applied multivariate curve decomposition analysis for our spectrum and recapitulated the results. Our imaging and analysis techniques will lead to the molecular brain atlas with single-cell resolution.

Domaines: Traitement du signal et de l'image [eess.SP]

Yusuke Murakami, Shinichi Miyazaki, Damien Boildieu, Zakaniaina Rajaofara, David Helbert, et al.. Toward Whole Brain Label-free Molecular Imaging with Single-cell Resolution sing Ultra-broadband Multiplex CARS Microspectroscopy. SPIE Photonics West, Jan 2022, San Francisco, California, United States. pp.71, ⟨10.1117/12.2626342⟩. ⟨hal-03531147⟩