Antioxidant activity and toxicity study of cerium oxide nanoparticles stabilized with innovative functional copolymers - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Advanced Healthcare Materials Année : 2021

Antioxidant activity and toxicity study of cerium oxide nanoparticles stabilized with innovative functional copolymers

Résumé

Oxidative stress, which is one of the main harmful mechanisms of pathologies including ischemic stroke, contributes to both neurons and endothelial cell damages, leading to vascular lesions. Although many antioxidants have been tested in preclinical studies, no treatment is currently available for stroke patients. Since cerium oxide nanoparticles (CNPs) exhibit remarkable antioxidant capacities, our objective is to develop an innovative coating to enhance CNPs biocompatibility without disrupting their antioxidant capacities or enhance their toxicity. This study reports the synthesis and characterization of functional polymers and their impact on the enzyme-like catalytic activity of CNPs. To study the toxicity and the antioxidant properties of CNPs for stroke and particularly endothelial damages, in vitro studies are conducted on a cerebral endothelial cell line (bEnd.3). Despite their internalization in bEnd.3 cells, coated CNPs are devoid of cytotoxicity. Microscopy studies report an intracellular localization of CNPs, more precisely in endosomes. All CNPs reduces glutamate-induced intracellular production of ROS in endothelial cells but one CNP significantly reduces both the production of mitochondrial superoxide anion and DNA oxidation. In vivo studies report a lack of toxicity in mice. This study therefore describes and identifies biocompatible CNPs with interesting antioxidant properties for ischemic stroke and related pathologies.
Fichier principal
Vignette du fichier
Goujon et al. to appear in Advanced Healthcare Materials version for ArXiv 07-Apr-21.pdf (11.17 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03452741 , version 1 (27-11-2021)

Identifiants

Citer

Geoffroy Goujon, Victor Baldim, Caroline Roques, Nicolas Bia, Johanne Seguin, et al.. Antioxidant activity and toxicity study of cerium oxide nanoparticles stabilized with innovative functional copolymers. Advanced Healthcare Materials, 2021, 10 (11), pp.2100059. ⟨10.1002/adhm.202100059⟩. ⟨hal-03452741⟩
125 Consultations
30 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More