Acyl-lipid metabolism

Yonghua Li-Beisson (1) , Basil Shorrosh , Fred Beisson (1) , Mats Andersson , Vincent Arondel , Philip Bates , Sébastien Baud , David Bird , Allan Debono , Timothy Durrett , Rochus Franke , Ian Graham , Kenta Katayama , Amélie Kelly , Tony Larson , Jonathan Markham , Martine Miquel , Isabel Molina , Ikuo Nishida , Owen Rowland , Lacey Samuels , Katherine Schmid , Hajime Wada , Ruth Welti , Changcheng Xu , Rémi Zallot , John Ohlrogge (2)

1 EBMP - Environnement, Bioénergie, Microalgues et Plantes
2 Department of Plant Biology - Michigan State University

Acyl lipids in Arabidopsis and all other plants have a myriad of diverse functions. These include providing the core diffusion barrier of the membranes that separates cells and subcellular organelles. This function alone involves more than 10 membrane lipid classes, including the phospholipids, galactolipids, and sphingolipids, and within each class the variations in acyl chain composition expand the number of structures to several hundred possible molecular species. Acyl lipids in the form of triacylglycerol account for 35% of the weight of Arabidopsis seeds and represent their major form of carbon and energy storage. A layer of cutin and cuticular waxes that restricts the loss of water and provides protection from invasions by pathogens and other stresses covers the entire aerial surface of Arabidopsis. Similar functions are provided by suberin and its associated waxes that are localized in roots, seed coats, and abscission zones and are produced in response to wounding. This chapter focuses on the metabolic pathways that are associated with the biosynthesis and degradation of the acyl lipids mentioned above. These pathways, enzymes, and genes are also presented in detail in an associated website (ARALIP: http://aralip.plantbiology.msu.edu/). Protocols and methods used for analysis of Arabidopsis lipids are provided. Finally, a detailed summary of the composition of Arabidopsis lipids is provided in three figures and 15 tables.

Domaines: Sciences du Vivant [q-bio]

Yonghua Li-Beisson, Basil Shorrosh, Fred Beisson, Mats Andersson, Vincent Arondel, et al.. Acyl-lipid metabolism. Arabidopsis Book, 2013, 11, pp.e0161. ⟨10.1199/tab.0161⟩. ⟨hal-03429781⟩