Oxidatively Generated Guanine(C8)-Thymine(N3) Intrastrand Cross-links in Double-stranded DNA Are Repaired by Base Excision Repair Pathways

Ibtissam Talhaoui (1) , Vladimir Shafirovich (2) , Zhi Liu (3) , Christine Saint-Pierre (4, 5) , Zhiger Akishev (6) , Bakhyt T Matkarimov (7) , Didier Gasparutto (4, 5) , Nicholas E Geacintov , Murat Saparbaev (1)

1 UMR 8200 - Stabilité Génétique et Oncogenèse
2 NYU - New York University [New York]
3 School of Communication and Information Engineering [Shanghai]
4 CREAB - Chimie pour la Reconnaissance et l’Etude d’Assemblages Biologiques
5 SPRAM - UMR 5819 - Structures et propriétés d'architectures moléculaire
6 KazNU - Al-Farabi Kazakh National University [Almaty]
7 National Laboratory Astana

Background: Base excision repair (BER) is the major pathway for repair of single oxidized nucleobases. Results: Bifunctional DNA glycosylases and AP endonucleases are able to remove cross-linked guanine in guanine(C8)-thymine(N3) intrastrand cross-links. Conclusion: BER pathways can repair the intrastrand cross-links. Significance: Oxidatively generated intrastrand cross-linked DNA lesions can be repaired in HeLa cell extracts not only by nucleotide excision repair, but also by multiple BER pathways.

Domaines: Sciences du Vivant [q-bio]

Ibtissam Talhaoui, Vladimir Shafirovich, Zhi Liu, Christine Saint-Pierre, Zhiger Akishev, et al.. Oxidatively Generated Guanine(C8)-Thymine(N3) Intrastrand Cross-links in Double-stranded DNA Are Repaired by Base Excision Repair Pathways. Journal of Biological Chemistry, 2015, 290, pp.14610 - 14617. ⟨10.1074/jbc.m115.647487⟩. ⟨hal-03419414⟩