Skip to Main content Skip to Navigation
Journal articles

Electrochemical and electronic detection of biomarkers in serum: a systematic comparison using aptamer-functionalized surfaces

Abstract : Sensitive and selective detection of biomarkers in serum in a short time has a significant impact on health. The enormous clinical importance of developing reliable methods and devices for testing serum levels of cardiac troponin I (cTnI), which are directly correlated to acute myocardial infarction (AMI), has spurred an unmatched race among researchers for the development of highly sensitive and cost-effective sensing formats to be able to differentiate patients with early onset of cardiac injury from healthy individuals with a mean cTnI level of 26 pg mL-1. Electronic- and electrochemical-based detection schemes allow for fast and quantitative detection not otherwise possible at the point of care. Such approaches rely largely on voltammetric and field-effect-based readouts. Here, we systematically investigate electric and electrochemical point-of-care sensors for the detection of cTnI in serum samples by using the same surface receptors, cTnI aptamer-functionalized CVD graphene-coated interdigated gold electrodes. The analytical performances of both sensors are comparable with a limit of detection (LoD) of 5.7 ± 0.6 pg mL-1(electrochemical) and 3.3 ± 1.2 pg mL-1 (electric). However, both sensors exhibit different equilibrium dissociation constant (KD) values between the aptamer-linked surface receptor and the cTnI analyte, being 160 pg mL-1 for the electrochemical and about three times lower for the electrical approach with KD = 51.4 pg mL-1. This difference is believed to be related to the use of a redox mediator in the electrochemical sensor for readout. The ability of the redox mediator to diffuse from the solution to the surface via the cTnI/aptamer interface is hindered, correlating to higher KD values. In contrast, the electric readout has the advantage of being label-free with a sensing limitation due to ionic strength effects, which can be limited using poly(ethylene) glycol surface ligands.
Document type :
Journal articles
Complete list of metadata
Contributor : Collection Iemn Connect in order to contact the contributor
Submitted on : Tuesday, October 26, 2021 - 11:19:09 AM
Last modification on : Tuesday, January 4, 2022 - 6:12:17 AM



Vladyslav Mishyn, Teresa Rodrigues, Yann R. Leroux, Laura Butruille, Eloise Woitrain, et al.. Electrochemical and electronic detection of biomarkers in serum: a systematic comparison using aptamer-functionalized surfaces. Analytical and Bioanalytical Chemistry, Springer Verlag, In press, ⟨10.1007/s00216-021-03658-0⟩. ⟨hal-03403532⟩



Les métriques sont temporairement indisponibles