Structural insight into the role of the PAS domain for signal transduction in sensor-kinase BvgS

Elian Dupré , Bernard Clantin , Youhua Yuan , Sophie Lecher , Elodie Lesne , Rudy Antoine , Vincent Villeret , Françoise Jacob-Dubuisson (1)

1 CIIL - Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204

The two-component system BvgAS controls the virulence regulon in Bordetella pertussis. BvgS is the prototype of a family of sensor histidine-kinases harboring periplasmic Venus flytrap (VFT) domains. The VFT domains are connected to the cytoplasmic kinase moiety by helical linkers separated by a Per-ARNT-Sim (PAS) domain. Antagonism between the two linkers, as one forms a coiled coil when the other is dynamic and vice versa, regulates BvgS activity. Here we solved the structure of the intervening PAS domain by X-ray

Mots clés: PAS domain of BvgS Two-component system; Bordetella pertussis; sensor-kinases; PAS domain; virulence regulation

Domaines: Bactériologie; Biologie structurale [q-bio.BM]

Elian Dupré, Bernard Clantin, Youhua Yuan, Sophie Lecher, Elodie Lesne, et al.. Structural insight into the role of the PAS domain for signal transduction in sensor-kinase BvgS. Journal of Bacteriology, 2021. ⟨hal-03401949⟩