Phase Correction for Absorption Mode Two-Dimensional Mass Spectrometry

Marc-André Delsuc (1, 2) , Kathrin Breuker (3) , Maria van Agthoven (3)

1 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire
2 CASC4DE - CASC4DE S.A.S
3 Leopold Franzens Universität Innsbruck - University of Innsbruck

Two-dimensional mass spectrometry (2D MS) is a tandem mass spectrometry method that relies on manipulating ion motions to correlate precursor and fragment ion signals. 2D mass spectra are obtained by performing a Fourier transform in both the precursor ion mass-to-charge ratio (m/z) dimension and the fragment ion m/z dimension. The phase of the ion signals evolves linearly in the precursor m/z dimension and quadratically in the fragment m/z dimension. This study demonstrates that phase-corrected absorption mode 2D mass spectrometry improves signal-to-noise ratios by a factor of 2 and resolving power by a factor of 2 in each dimension compared to magnitude mode. Furthermore, phase correction leads to an easier differentiation between ion signals and artefacts, and therefore easier data interpretation.

Mots clés: absorption mode; data processing; mass spectrometry; phase correction; tandem mass spectrometry; two-dimensional

Domaines: Chimie

Marc-André Delsuc, Kathrin Breuker, Maria van Agthoven. Phase Correction for Absorption Mode Two-Dimensional Mass Spectrometry. Molecules, 2021, 26 (11), pp.3388. ⟨10.3390/molecules26113388⟩. ⟨hal-03372399⟩