The Physcomitrium (Physcomitrella) patens PpKAI2L receptors for strigolactones and related compounds function via MAX2-dependent and independent pathways

Mauricio Lopez-Obando (1, 2, 3) , Ambre Guillory (1) , François-Didier Boyer (4) , David Cornu (5) , Beate Hoffmann (1) , Philippe Le Bris (1) , Jean-Bernard Pouvreau (6) , Philippe Delavault (6) , Catherine Rameau (1) , Alexandre de Saint Germain (1) , Sandrine Bonhomme (1)

1 IJPB - Institut Jean-Pierre Bourgin
2 SLU - Swedish University of Agricultural Sciences = Sveriges lantbruksuniversitet
3 Vedas Recherche et Innovation (Vedas CII)
4 ICSN - Institut de Chimie des Substances Naturelles
5 I2BC - Institut de Biologie Intégrative de la Cellule
6 LBPV - Laboratoire de biologie et pathologie végétales

In angiosperms, the a/b hydrolase DWARF14 (D14), along with the F-box protein MORE AXILLARY GROWTH2 (MAX2), perceives strigolactones (SL) to regulate developmental processes. The key SL biosynthetic enzyme CAROTENOID CLEAVAGE DIOXYGENASE8 (CCD8) is present in the moss Physcomitrium patens, and PpCCD8-derived compounds regulate moss extension. The PpMAX2 homolog is not involved in the SL response, but 13 PpKAI2LIKE (PpKAI2L) genes homologous to the D14 ancestral paralog KARRIKIN INSENSITIVE2 (KAI2) encode candidate SL receptors. In Arabidopsis thaliana, AtKAI2 perceives karrikins and the elusive endogenous KAI2-Ligand (KL). Here, germination assays of the parasitic plant Phelipanche ramosa suggested that PpCCD8-derived compounds are likely non-canonical SLs. (+)-GR24 SL analog is a good mimic for PpCCD8-derived compounds in P. patens, while the effects of its enantiomer (-)-GR24, a KL mimic in angiosperms, are minimal. Interaction and binding assays of seven PpKAI2L proteins pointed to the stereoselectivity towards (-)-GR24 for a single clade of PpKAI2L (eu-KAI2). Enzyme assays highlighted the peculiar behavior of PpKAI2L-H. Phenotypic characterization of Ppkai2l mutants showed that eu-KAI2 genes are not involved in the perception of PpCCD8derived compounds but act in a PpMAX2-dependent pathway. By contrast, mutations in PpKAI2L-G, and-J genes abolished the response to the (+)-GR24 enantiomer, suggesting that PpKAI2L-G, and-J proteins are receptors for moss SLs.

Domaines: Botanique; Biochimie, Biologie Moléculaire

Mauricio Lopez-Obando, Ambre Guillory, François-Didier Boyer, David Cornu, Beate Hoffmann, et al.. The Physcomitrium (Physcomitrella) patens PpKAI2L receptors for strigolactones and related compounds function via MAX2-dependent and independent pathways. The Plant cell, 2021, 33, pp.3487-3512. ⟨10.1093/plcell/koab217⟩. ⟨hal-03366148⟩