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Article Dans Une Revue Optics Express Année : 2022

Computational hyperspectral light-sheet microscopy

Résumé

We describe a computational light-sheet microscope designed for hyperspectral acquisition at high spectral resolution. The fluorescence light emitted from the full field-of-view is focused along the entrance slit of an imaging spectrometer using a cylindrical lens. To acquire the spatial dimension orthogonal to the slit of the spectrometer, we propose to illuminate the specimen with a sequence of structured light patterns and to solve the image reconstruction problem. Beam shaping is obtained simply using a digital micromirror device in conjunction with a traditional selective plane illumination microscopy setup. We demonstrate the feasibility of this method and report the first results in vivo in hydra specimens labeled using two fluorophores.
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Dates et versions

hal-03363493 , version 1 (04-10-2021)
hal-03363493 , version 2 (01-02-2022)

Identifiants

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Sébastien Crombez, Pierre Leclerc, Cédric Ray, Nicolas Ducros. Computational hyperspectral light-sheet microscopy. Optics Express, 2022, 30 (4), pp.4856-4866. ⟨10.1364/OE.442043⟩. ⟨hal-03363493v2⟩
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