Maternal age alters gene expression in blastocyst trophoblast and term placenta as well as term placental structure

Emilie Derisoud (1) , Sarah Gastaldo (1) , Orianne Valais (1) , Luc Jouneau (1) , Cedric Dubois (2) , Alexia Durand (1) , Audrey Prézelin (1) , Michèle Dahirel (1) , Yann Jaszczyszyn (3) , Laurence Wimel (2) , Pascale Chavatte-Palmer (1)

1 BREED - Biologie de la Reproduction, Environnement, Epigénétique & Développement
2 IFCE - Institut Français du Cheval et de l'Equitation [Saumur]
3 PF I2BC - Département Plateforme

Possibly contribute to the lower fertility in older mares Gene expression & histology of placentas are affected by maternal age but no gross morphology Possibly affects fetomaternal communication/exchanges Perturbations in gene expression in placentas are already present in the embryo trophoblast RNAseq differential analysis: DESeq2 package on R software with size and sex as cofactors for embryos; gestation length, foal sex and placental weight as cofactors for placentas; padj<0.05 for significance Gene Set Enrichment Analysis (GSEA): Broad Institute software using the KEGG and GO biological process (BP) databases → Redundancy was reduced using SUMER analysis FDR<0.05 for significance; In analyses, "young" is the reference group Stereological analysis: Microcotyledonary and allantoid relative surface and volume quantification by One Stop Stereology using Mercator® software (ExploraNova) Statistics: linear model with permutations with R software, including gestation length, mare weight and foal sex

Mots clés: Mare; embryo; placenta; trophoblast; RNA-seq

Domaines: Médecine vétérinaire et santé animale

Emilie Derisoud, Sarah Gastaldo, Orianne Valais, Luc Jouneau, Cedric Dubois, et al.. Maternal age alters gene expression in blastocyst trophoblast and term placenta as well as term placental structure. IFPA meeting 2021, Aug 2021, online, Netherlands. . ⟨hal-03348714⟩