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Amphipathic environments for determining the structure of membrane proteins by single-particle electron cryo-microscopy

Abstract : Over the past decade, the structural biology of membrane proteins (MPs) has taken a new turn thanks to epoch-making technical progress in single-particle electron cryo-microscopy (cryo-EM) as well as to improvements in sample preparation. The present analysis provides an overview of the extent and modes of usage of the various types of surfactants for cryo-EM studies. Digitonin, dodecylmaltoside, protein-based nanodiscs, lauryl maltoside-neopentyl glycol, glyco-diosgenin and amphipols are the most popular surfactants at the vitrification step. Surfactant exchange is frequently used between MP purification and grid preparation, requiring extensive optimization each time the study of a new MP is undertaken. The variety of both the surfactants and experimental approaches used over the past few years bears witness to the need to continue developing innovative surfactants and optimizing conditions for sample preparation. The possibilities offered by novel amphipols for EM applications are discussed.
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https://hal.archives-ouvertes.fr/hal-03322455
Contributor : Manuela Zoonens Connect in order to contact the contributor
Submitted on : Thursday, August 19, 2021 - 10:45:58 AM
Last modification on : Tuesday, September 28, 2021 - 5:16:44 PM
Long-term archiving on: : Saturday, November 20, 2021 - 6:49:49 PM

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Christel Le Bon, Baptiste Michon, Jean-Luc Popot, M. Zoonens. Amphipathic environments for determining the structure of membrane proteins by single-particle electron cryo-microscopy. Quarterly Reviews of Biophysics, Cambridge University Press (CUP), 2021, 54, pp.e6. ⟨10.1017/S0033583521000044⟩. ⟨hal-03322455⟩

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