Gold(III) porphyrins: Synthesis and interaction with G-quadruplex DNA

Tiffany Rundstadler (1) , Emmanuelle Mothes (1) , Samir Amrane (2, 3) , Jean-Luc Stigliani (1) , Pierre Verhaeghe (1) , G. Pratviel (1)

1 LCC - Laboratoire de chimie de coordination
2 UB - Université de Bordeaux
3 ARNA - Acides Nucléiques : Régulations Naturelle et Artificielle

G-quadruplex nucleic acids (G4s) are RNA and DNA secondary structures involved in the regulation of multiple key biological processes. They can be found in telomeres, oncogene promoters, RNAs, but also in viral genomes. Due to their unique structural features, very distinct from the canonical duplexes or single-strands, G4s represent promising pharmacological targets for small molecules, namely G4-ligands. Gold(III) penta-cationic porphyrins, as specific G4 ligands, are able to inhibit HIV-1 infectivity and their antiviral activity correlates with their affinity for G4s. Up to now, one of the best antiviral compounds is meso-5,10,15,20-tetrakis[4-(N-methyl-pyridinium-2-yl)phenyl]porphyrinato gold(III) (1). Starting from this compound, we report a structure/affinity relationship study of gold(III) cationic porphyrins to find out the best porphyrin candidate for functionalization, in order to study the antiviral mechanism of action of these gold(III) porphyrins.

Mots clés: Au3+-complexes; FRET melting; HIV-1; Cationic porphyrins; Modeling; G4

Domaines: Chimie de coordination

Tiffany Rundstadler, Emmanuelle Mothes, Samir Amrane, Jean-Luc Stigliani, Pierre Verhaeghe, et al.. Gold(III) porphyrins: Synthesis and interaction with G-quadruplex DNA. Journal of Inorganic Biochemistry, 2021, 223, pp.111551. ⟨10.1016/j.jinorgbio.2021.111551⟩. ⟨hal-03312437⟩