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Cell–cell interactions via non-covalent click chemistry

Abstract : Metabolic glycoengineering with unnatural sugars became a valuable tool for introducing recognition markers on the cell membranes via bioorthogonal chemistry. By using this strategy, we functionalized the surface of tumor and T cells using complementary artificial markers based on both β-cyclodextrins (β-CDs) and adamantyl trimers, respectively. Once tied on cell surfaces, the artificial markers induced cell–cell adhesion through non-covalent click chemistry. These unnatural interactions between A459 lung tumor cells and Jurkat T cells triggered the activation of natural killer (NK) cells thanks to the increased production of interleukin-2 (IL-2) in the vicinity of cancer cells, leading ultimately to their cytolysis. The ready-to-use surface markers designed in this study can be easily inserted on the membrane of a wide range of cells previously submitted to metabolic glycoengineering, thereby offering a simple way to investigate and manipulate intercellular interactions.
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Submitted on : Friday, August 27, 2021 - 2:57:34 PM
Last modification on : Wednesday, October 20, 2021 - 3:22:22 AM


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Chad Plumet, Achmet Said Mohamed, Tanguy Vendeuvre, Brigitte Renoux, Jonathan Clarhaut, et al.. Cell–cell interactions via non-covalent click chemistry. Chemical Science , The Royal Society of Chemistry, 2021, 12 (26), pp.9017-9021. ⟨10.1039/D1SC01637G⟩. ⟨hal-03285569⟩



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