Phospholipid transfer to high-density lipoprotein (HDL) upon triglyceride lipolysis is directly correlated with HDL-cholesterol levels and is not associated with cardiovascular risk

Feng Ma (1) , Maryam Darabi (1) , Marie Lhomme (1) , Emilie Tubeuf (1) , Aurélie Canicio (1) , Jean Brerault (1) , Narcisse Medadje (1) , Fabiana Rached (1) , Sandrine Lebreton (2) , Eric Frisdal (1) , Fernando Brites (1) , Carlos Serrano (1) , Raul Santos (1) , Emmanuel Gautier (1) , Thierry Huby (1) , Petra El Khoury (1) , Alain Carrié (1) , Marianne Abifadel (1) , Eric Bruckert (1) , Maryse Guerin (1) , Philippe Couvert (1) , Philippe Giral (1) , Philippe Lesnik (1) , Wilfried Le Goff (1) , Isabelle Guillas (1) , Anatol Kontush (1)

1 ICAN - Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases
2 iEES Paris - Institut d'écologie et des sciences de l'environnement de Paris

Background and aims While low concentrations of high-density lipoprotein-cholesterol (HDL-C) represent a well-established cardiovascular risk factor, extremely high HDL-C is paradoxically associated with elevated cardiovascular risk, resulting in the U-shape relationship with cardiovascular disease. Free cholesterol transfer to HDL upon lipolysis of triglyceride-rich lipoproteins (TGRL) was recently reported to underlie this relationship, linking HDL-C to triglyceride metabolism and atherosclerosis. In addition to free cholesterol, other surface components of TGRL, primarily phospholipids, are transferred to HDL during lipolysis. It remains indeterminate as to whether such transfer is linked to HDL-C and cardiovascular disease. Methods and results When TGRL was labelled with fluorescent phospholipid 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI), time- and dose-dependent transfer of DiI to HDL was observed upon incubations with lipoprotein lipase (LPL). The capacity of HDL to acquire DiI was decreased by −36% (p<0.001) in low HDL-C patients with acute myocardial infarction (n = 22) and by -95% (p<0.001) in low HDL-C subjects with Tangier disease (n = 7), unchanged in low HDL-C patients with Type 2 diabetes (n = 17) and in subjects with high HDL-C (n = 20), and elevated in subjects with extremely high HDL-C (+11%, p<0.05) relative to healthy normolipidemic controls. Across all the populations combined, HDL capacity to acquire DiI was directly correlated with HDL-C (r = 0.58, p<0.001). No relationship of HDL capacity to acquire DiI with both overall and cardiovascular mortality obtained from epidemiological studies for the mean HDL-C levels observed in the studied populations was obtained. Conclusions These data indicate that the capacity of HDL to acquire phospholipid from TGRL upon LPL-mediated lipolysis is proportional to HDL-C and does not reflect cardiovascular risk in subjects widely differing in HDL-C levels.

Mots clés: Phospholipid; Lipolysis; HDL; Triglyceride-rich lipoprotein; LPL

Domaines: Alimentation et Nutrition

Feng Ma, Maryam Darabi, Marie Lhomme, Emilie Tubeuf, Aurélie Canicio, et al.. Phospholipid transfer to high-density lipoprotein (HDL) upon triglyceride lipolysis is directly correlated with HDL-cholesterol levels and is not associated with cardiovascular risk. Atherosclerosis, 2021, 324, pp.1-8. ⟨10.1016/j.atherosclerosis.2021.03.002⟩. ⟨hal-03280868⟩