Skip to Main content Skip to Navigation
Journal articles

Isolating intact phytoplasma particles from plants for sequencing applications and for testing host responses to phytoplasmas

Abstract : Candidatus Phytoplasma species are obligate plant parasites limited to the phloem tissue. The difficulties to grow phytoplasma in culture seriously impedes the study of these prokaryotes. In this study, a protocol to extract intact phytoplasmas from infected periwinkle plants using filtration, differential and density-gradient centrifugations was optimized. Confocal and transmission electron microscopy was used to visualize intact phytoplasmas in the preparation. Quantitative-PCR and DNA-seq analyses revealed that phytoplasma DNA was significantly enriched following the extraction procedure. Challenging periwinkle plants with phytoplasma preparation induced the expression of one defence-related gene. This study provides an optimized procedure for phytoplasma DNA enrichment and for extracting intact phytoplasmas that can be used to examine host responses to them.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-03270218
Contributor : Diego Santos Garcia <>
Submitted on : Tuesday, August 31, 2021 - 4:56:25 PM
Last modification on : Tuesday, August 31, 2021 - 5:08:40 PM

File

IPWG2019_OBahar_2_DSG.pdf
Files produced by the author(s)

Identifiers

Citation

Nofar Assoline, Diego Santos-Garcia, Vered Naor, Ofir Bahar. Isolating intact phytoplasma particles from plants for sequencing applications and for testing host responses to phytoplasmas. Phytopathogenic Mollicutes, Technology Society Of Basic And Applied Sciences, 2019, 9 (1), pp.125-126. ⟨10.5958/2249-4677.2019.00063.X⟩. ⟨hal-03270218⟩

Share

Metrics

Record views

19

Files downloads

10