Analytical comparison of ELISA and mass spectrometry for quantification of serum hepcidin in critically ill patients

Constance Delaby (1, 2, 3, 4, 5) , Jérôme Vialaret (3, 4, 5) , Christophe Hirtz (4, 5) , Thibaud Lefebvre (6, 7, 8) , Matthias Herkert , Hervé Puy (6, 7, 8) , Sigismond Lasocki (9) , Pierre Asfar , Alain Mercat , Thomas Gaillard , Soizic Gergaud , Cyrille Sargentini , Claire Geneve , Philippe Montravers , Thibault Lefebvre , Grégoire Mercier , Nicolas Nagot (10) , Sylvain Lehmann (4, 5) , Gerald Chanques (11, 5) , Samir Jaber (11, 5) , Karim Asehnoune , Antoine Roquilly , Claire Dahyot-Fizelier , Olivier Mimoz , Sonia Isslame , Philippe Seguin , Mathilde Barbaz , Martine Ferrandiere , Thomas Kerforne , Katell Peoc'H , François Beloncle , Maxime Leger , Emmanuel Rineau , Gregory Marin , Matthieu Boisson , Yoann Launey

1 Hospital de la Santa Creu i Sant Pau
2 UAB - Universitat Autònoma de Barcelona = Autonomous University of Barcelona = Universidad Autónoma de Barcelona
3 INM - Institut des Neurosciences de Montpellier
4 Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB)
5 CHRU Montpellier - Centre Hospitalier Régional Universitaire [Montpellier]
6 CRI (UMR_S_1149 / ERL_8252 / U1149) - Centre de recherche sur l'Inflammation
7 Labex Gr-Ex - Laboratoire d'Excellence : Biogenèse et pathologies du globule rouge
8 Hôpital Louis Mourier - AP-HP [Colombes]
9 MITOVASC - MitoVasc - Physiopathologie Cardiovasculaire et Mitochondriale
10 PCCEI - Pathogenesis and Control of Chronic and Emerging Infections
11 PhyMedExp - Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046]

Aim: To compare methods of quantifying serum hepcidin (based on MS and ELISA) and their ability to diagnose true iron deficiency anemia in critically ill patients. Materials & methods: Serum hepcidin was measured in 119 critically ill patients included in the HEPCIDANE clinical trial, using either an ultra-sensitive ELISA kit (from DRG) or two different MS methods. Results: The results show a good correlation between the different methods studied. The Bland–Altman analysis and the Kappa test for clinical groups show a good or very good agreement between the different tests. Conclusion: ELISA or MS show a satisfactory commutability to quantify serum hepcidin. This is of great importance for the determination of therapeutic strategies in iron deficiency.

Domaines: Sciences du Vivant [q-bio]

Constance Delaby, Jérôme Vialaret, Christophe Hirtz, Thibaud Lefebvre, Matthias Herkert, et al.. Analytical comparison of ELISA and mass spectrometry for quantification of serum hepcidin in critically ill patients. Bioanalysis, inPress, ⟨10.4155/bio-2021-0069⟩. ⟨hal-03258593⟩