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Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni
Abstract : Automated ChIPmentation procedure is a convenient alternative to native chromatin immunoprecipitation (N-ChIP). It is now routinely used for ChIP-Seq. Using the human parasite Schistosoma mansoni , whose production requires scarifying animals and should therefore kept to a minimum, we show here that the automated ChIPmentation is suitable for limited biological material. We define as operational limit ≥20,000 cells. We also present a streamlined protocol for the preparation of ChIP input libraries.
https://hal.archives-ouvertes.fr/hal-03255320
Contributor : Anne Modat Connect in order to contact the contributor
Submitted on : Thursday, April 14, 2022 - 11:08:51 AM
Last modification on : Tuesday, May 17, 2022 - 2:30:06 PM
Contributor : Anne Modat Connect in order to contact the contributor
Submitted on : Thursday, April 14, 2022 - 11:08:51 AM
Last modification on : Tuesday, May 17, 2022 - 2:30:06 PM
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