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Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni

Abstract : Automated ChIPmentation procedure is a convenient alternative to native chromatin immunoprecipitation (N-ChIP). It is now routinely used for ChIP-Seq. Using the human parasite Schistosoma mansoni , whose production requires scarifying animals and should therefore kept to a minimum, we show here that the automated ChIPmentation is suitable for limited biological material. We define as operational limit ≥20,000 cells. We also present a streamlined protocol for the preparation of ChIP input libraries.
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https://hal.archives-ouvertes.fr/hal-03255320
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Submitted on : Thursday, April 14, 2022 - 11:08:51 AM
Last modification on : Tuesday, May 17, 2022 - 2:30:06 PM

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Chrystelle Lasica, Ronaldo de Carvalho Augusto, Hélène Moné, Gabriel Mouahid, Cristian Chaparro, et al.. Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni. Wellcome Open Research, F1000Research, 2022, 7, pp.133. ⟨10.12688/wellcomeopenres.17779.1⟩. ⟨hal-03255320v2⟩

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