Catalysis of the electrochemical oxygen reduction reaction (ORR) by animal and human cells

Simon Guette-Marquet (1) , Christine Roques (1) , Alain Bergel (1)

1 LGC - Laboratoire de Génie Chimique

Animal cells from the Vero lineage and MRC5 human cells were checked for their capacity to catalyse the electrochemical oxygen reduction reaction (ORR). The Vero cells needed 72 hours’ incubation to induce ORR catalysis. The cyclic voltammetry curves were clearly modified by the presence of the cells with a shift of ORR of 50 mV towards positive potentials and the appearance of a limiting current (59 μA.cm-2). The MRC5 cells induced considerable ORR catalysis after only 4 h of incubation with a potential shift of 110 mV but with large experimental deviation. A longer incubation time, of 24 h, made the results more reproducible with a potential shift of 90 mV. The presence of carbon nanotubes on the electrode surface or pre-treatment with foetal bovine serum or poly-D-lysine did not change the results. These data are the first demonstrations of the capability of animal and human cells to catalyse electrochemical ORR. The discussion of the possible mechanisms suggests that these pioneering observations could pave the way for electrochemical biosensors able to characterize the protective system of cells against oxidative stress and its sensitivity to external agents.

Mots clés: MRC-5; Vero; Electrochemistry; Cyclic voltammetry -Biocathode; Oxidative stress; Antioxydant enzymes

Domaines: Génie chimique; Catalyse; Biotechnologies

Simon Guette-Marquet, Christine Roques, Alain Bergel. Catalysis of the electrochemical oxygen reduction reaction (ORR) by animal and human cells. PLoS ONE, 2021, 16 (5), pp.e0251273. ⟨10.1371/journal.pone.0251273⟩. ⟨hal-03254630⟩