A role for erythrocyte band 3 degradation by the parasite gp76 serine protease in the formation of the parasitophorous vacuole during invasion of erythrocytes by Plasmodium falciparum

Emmanuelle Roggwiller (1) , Maria Rugenia Morales Bétoulle (1) , Thierry Blisnick (1) , Catherine Braun Breton (1)

1 Parasitologie Expérimentale

A purified Plasmodium falciparum serine protease (gp76) implicated in erythrocyte invasion, degrades human erythrocyte band 3 and glycophorin A. Inhibition studies using synthetic peptides derived from the presumed band 3 enzymatic cleavage sites and the observed uptake of fluorescent phospholipids following gp76 treatment, suggest that band 3 degradation by this serine protease participates in the formation of the parasitophorous vacuole by restructuring the red cell cytoskeleton. These results provide a rationale for the elaboration of specific inhibitors to block red cell invasion by malaria parasites.

Domaines: Microbiologie et Parasitologie

Emmanuelle Roggwiller, Maria Rugenia Morales Bétoulle, Thierry Blisnick, Catherine Braun Breton. A role for erythrocyte band 3 degradation by the parasite gp76 serine protease in the formation of the parasitophorous vacuole during invasion of erythrocytes by Plasmodium falciparum. Molecular and Biochemical Parasitology, 1996, 82 (1), pp.13-24. ⟨10.1016/0166-6851(96)02714-4⟩. ⟨hal-03246345⟩