PDZ Sample Quality Assessment by Biochemical and Biophysical Characterizations

Célia Caillet-Saguy (1) , Sébastien Brûlé (2) , Nicolas Wolff (1) , Bertrand Raynal (2)

1 Récepteurs Canaux - Channel Receptors
2 Biophysique Moléculaire (plateforme) - Molecular Biophysics (platform)

PDZ domains are small globular domains involved in protein–protein interactions. They participate in a wide range of critical cellular processes. These domains, very abundant in the human proteome, are widely studied by high-throughput interactomics approaches and by biophysical and structural methods. However, the quality of the results is strongly related to the optimal folding and solubility of the domains. We provide here a detailed description of protocols for a strict quality assessment of the PDZ constructs. We describe appropriate experimental approaches that have been selected to overcome the small size of such domains to check the purity, identity, homogeneity, stability, and folding of samples.

Mots clés: PDZ domain; Folding; Protein sample; Quality control; Biophysical techniques; Stability; Purity; Identity; Structure; Quantification

Domaines: Sciences du Vivant [q-bio]

Célia Caillet-Saguy, Sébastien Brûlé, Nicolas Wolff, Bertrand Raynal. PDZ Sample Quality Assessment by Biochemical and Biophysical Characterizations. Jean-Paul Borg. PDZ Mediated Interactions : methods and protocols, 2256, Humana Press, pp.89-124, 2021, Methods in molecular biology, 978-1-0716-1166-1 (ebook). ⟨10.1007/978-1-0716-1166-1_6⟩. ⟨hal-03234405⟩