An ultra-high bandwidth nano-electronic interface to the interior of living cells with integrated fluorescence readout of metabolic activity

Dandan Ren (1) , Zahra Nemati (2) , Chia-Hung Lee (2) , Jinfeng Li (2) , Kamel Haddadi (3) , Douglas C. Wallace (4) , Peter J. Burke (2)

1 ECE - UC San Diego - Department of Electrical and Computer Engineering [Univ California San Diego]
2 UC Irvine - University of California [Irvine]
3 CSAM - IEMN - Circuits Systèmes Applications des Micro-ondes - IEMN
4 University of Pennsylvania

We present the first ever broadband, calibrated electrical connection to the inside of a cell. The interior of a vital, living cell contains multiple dynamic and electrically active organelles such as mitochondria, chloroplasts, lysosomes, and the endoplasmic reticulum. However, little is known about the detailed electrical activity inside the cell. Here we show an ultra-high bandwidth nano-electronic interface to the interior of living cells with integrated fluorescence readout of metabolic activity. On-chip/on-petri dish nanoscale capacitance calibration standards are used to quantify the electronic coupling from bench to cell from DC to 26 GHz (with cell images at 22 GHz). The interaction of static to high frequency electromagnetic fields with the cell constituents induce currents of free charges and local reorganization of linked charges. As such, this enables a direct, calibrated, quantitative, nanoscale electronic interface to the interior of living cells. The interface could have a variety of applications in interfacing life sciences to nano-electronics, including electronic assays of membrane potential dynamics, nano-electronic actuation of cellular activity, and tomographic, nano-radar imaging of the morphology of vital organelles in the cytoplasm, during all phases of the cell life cycle (from development to senescence), under a variety of physiological environments, and under a broad suite of pharmacological manipulations.

Domaines: Micro et nanotechnologies/Microélectronique; Organisation et fonctions cellulaires [q-bio.SC]

Dandan Ren, Zahra Nemati, Chia-Hung Lee, Jinfeng Li, Kamel Haddadi, et al.. An ultra-high bandwidth nano-electronic interface to the interior of living cells with integrated fluorescence readout of metabolic activity. Scientific Reports, 2020, 10 (1), 10756, 12 p. ⟨10.1038/s41598-020-67408-5⟩. ⟨hal-03224644⟩