Application of NMR Spectroscopy to Determine the 3D Structure of Small Non-Coding RNAs

Marie-Eve Chagot (1) , Marc Quinternet (2) , Xavier Manival (1) , Isabelle Lebars (3)

1 IMoPA - Ingénierie Moléculaire et Physiopathologie Articulaire
2 IBSLor - Ingénierie, Biologie et Santé en Lorraine
3 ARN - Architecture et Réactivité de l'ARN

Many RNA architectures were discovered to be involved in a wide range of essential biological processes in all organisms from carrying genetic information to gene expression regulation. The remarkable ability of RNAs to adopt various architectures depending on their environment enables the achievement of their myriads of biological functions. Nuclear Magnetic Resonance (NMR) is a powerful technique to investigate both their structure and dynamics. NMR is also a key tool for studying interactions between RNAs and their numerous partners such as small molecules, ions, proteins, or other nucleic acids. In this chapter, to illustrate the use of NMR for 3D structure determination of small noncoding RNA, we describe detailed methods that we used for the yeast C/D box small nucleolar RNA U14 from sample preparation to 3D structure calculation.

Mots clés: RNA; NMR; 3D structure; dynamics; RNA

Domaines: Biologie structurale [q-bio.BM]

Marie-Eve Chagot, Marc Quinternet, Xavier Manival, Isabelle Lebars. Application of NMR Spectroscopy to Determine the 3D Structure of Small Non-Coding RNAs. Mathieu Rederstorff. Small Non-Coding RNAs. Methods and Protocols, 2300, Springer, pp.251-266, 2021, Methods in Molecular Biology, ⟨10.1007/978-1-0716-1386-3_19⟩. ⟨hal-03203667⟩