Are bacteria claustrophobic? The problem of micrometric spatial confinement for the culturing of micro-organisms

Céline Molinaro (1) , Violette da Cunha (2) , Aurore Gorlas (2) , François Iv (1) , Laurent Gallais (3) , Ryan Catchpole (2) , Patrick Forterre (2) , Guillaume Baffou (1)

1 MOSAIC - MOSAIC
2 I2BC - Institut de Biologie Intégrative de la Cellule
3 ILM - ILM

Culturing cells confined in microscale geometries has been reported in many studies this last decade, in particular following the development of microfluidic-based applications and lab-on-a-chip devices. Such studies usually examine growth of Escherichia coli. In this article, we show that E. coli may be a poor model and that spatial confinement can severely prevent the growth of many microorganisms. By studying different bacteria and confinement geometries, we determine that the growth inhibition observed for some bacteria results from fast dioxygen depletion, inherent to spatial confinement, and not to any depletion of nutriments. This article unravels the physical origin of confinement problems in cell culture, highlighting the importance of oxygen depletion, and paves the way for the effective culturing of bacteria in confined geometries by demonstrating enhanced cell growth in confined geometries in the proximity of air bubbles.

Domaines: Bactériologie; Organisation et fonctions cellulaires [q-bio.SC]

Céline Molinaro, Violette da Cunha, Aurore Gorlas, François Iv, Laurent Gallais, et al.. Are bacteria claustrophobic? The problem of micrometric spatial confinement for the culturing of micro-organisms. RSC Advances, 2021, 11 (21), pp.12500-12506. ⟨10.1039/d1ra00184a⟩. ⟨hal-03186330⟩