Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions

Lukas Gajdos (1, 2) , Matthew P Blakeley (2) , Atul Kumar (3) , Michaela Wimmerová (3) , Michael Haertlein (2) , V. Trevor Trevor Forsyth (2) , Anne Imberty (1) , Juliette M Devos (2)

1 CERMAV - Centre de Recherches sur les Macromolécules Végétales
2 ILL - Institut Laue-Langevin
3 CEITEC MU - Central European Institute of Technology [Brno]

Highlights d Joint X-ray/neutron structure of bacterial lectin in apo and complex form d Perdeuterated lectin and sugar improve the quality of neutron density maps d Fucose methine and methyl groups are involved in CH-p interactions with tryptophan d Room temperature data avoid glycerol-induced alternate loop conformations

Domaines: Chimie

Lukas Gajdos, Matthew P Blakeley, Atul Kumar, Michaela Wimmerová, Michael Haertlein, et al.. Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions. Structure, 2021, ⟨10.1016/j.str.2021.03.003⟩. ⟨hal-03180319⟩