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Exploitation of β -glycosyl azides for the preparation of α -glycosynthases

Abstract : An interesting approach for the chemo-enzymatic synthesis of carbohydrates is the use of glycosynthases, a class of mutant glycosidases derived from β-glycoside hydrolases obtained by replacement of the active site nucleophile with a non-nucleophilic residue. However, the scarcity of α-glycosynthases has so far hampered access to the synthesis of a large class of oligosaccharides of biotechnological interest. We review here a new glycosynthetic methodology for the production of two retaining α-fucosynthases and an α-galactosynthase exploiting β-glycosyl azide derivatives. The general applicability of this approach, which opens new perspectives in the use of azide derivatives for the production of novel α-glycosynthases, is discussed.
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https://hal.archives-ouvertes.fr/hal-03160483
Contributor : Gerlind Sulzenbacher Connect in order to contact the contributor
Submitted on : Thursday, March 25, 2021 - 4:24:06 PM
Last modification on : Thursday, January 27, 2022 - 3:31:05 AM

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Beatrice Cobucci-Ponzano, Carmela Zorzetti, Andrea Strazzulli, Emiliano Bedini, Maria Michela Corsaro, et al.. Exploitation of β -glycosyl azides for the preparation of α -glycosynthases. Biocatalysis and Biotransformation, Taylor & Francis, 2012, ⟨10.3109/10242422.2012.679814⟩. ⟨hal-03160483⟩

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